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Collect your biological sample. This could be a plant, animal or microorganism. Put the sample into a sterile tube, making sure not to contaminate it.

Utilize a lysis buffer to break down the cell walls and membranes. Stir or vortex to ensure an effective reaction. It's crucial not to overheat the sample, as this could denature the DNA.

Look to separate the DNA from proteins and other cellular debris. This is done through centrifugation. Be sure to pipette slowly to avoid damaging the DNA.

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